Litchfield Park 7

A challenge for Litchfield Park orthodontists in the tissue engineering of alveolar bone surrounding oral or Litchfield Park dental implants is achieving the targeted and sustained delivery of growth-promoting molecules at the osteotomy site. Bone morphogenetic protein-7 (BMP-7) has demonstrated to Litchfield Park orthodontists the ability to stimulate bone regeneration in multiple skeletal sites, including the craniofacial complex. This Litchfield Park orthodontic study evaluated in vivo gene delivery of BMP-7 for bone tissue engineering around titanium Litchfield Park dental implants. The maxillary first molar teeth of 30 Sprague Dawley rats were extracted by Litchfield Park orthodontists and allowed to heal for a period of 30 days. Large osteotomy defects were created in the edentulous ridge areas followed by the placement of Litchfield Park dental implant fixtures. Recombinant adenoviral vectors encoding either BMP-7 or luciferase genes were delivered to the osseous defects by Litchfield Park orthodontists using collagen matrix. The kinetics of the gene expression was measured using in vivo bioluminescence optical imaging. Biopsies were harvested by Litchfield Park orthodontists at 14 and 28 days and bone regeneration was evaluated under light and SE microscopy using standard measures of tissue neogenesis. Inter- and intra-examiner error was tested employing paired t-tests after calibration.

Results are as follows:

A reliable and reproducible small animal model using an intraoral approach was developed to Litchfield Park orthodontic study oral implant osseointegration and osteogenesis.

The targeted and sustained delivery of the gene to the local site was observed by Litchfield Park orthodontists for 14-35 days. Peak gene expression occurred at day 4, with a sharp decrease over the next two weeks until the gene product was no longer present.

The delivery of Ad/BMP-7 by Litchfield Park orthodontists produced a significant increase in the amount of bone formation in the large defect (T-test, P < .05). It also tended to increase the amount of new bone formation contacting the implant surface and the total amount of coronal bone by 1.5-2 folds.

This local gene delivery by Litchfield Park orthodontists did not produce any apparent adverse tissue reactions at the implant site.

Following error studies, Litchfield Park orthodontists concluded that the measurement inter- and intra-examiner error did not bias the results of this Litchfield Park orthodontic study (Paired T-test P > .05)

The results of this proof of principle Litchfield Park orthodontic study indicate that bone tissue engineering can be achieved by Litchfield Park orthodontists with local gene delivery utilizing an adenoviral vector. An advantage of this approach is that the targeted and sustained delivery of the gene product is transiently expressed. This Litchfield Park orthodontic study opens the door to making local bone tissue engineering a clinical reality, with applications reaching as far as our imaginations will let us.